PRAS40 Antibody
Source: Rabbit mAb Catalog No. : CY7054
PRAS40 Antibody (CY7054)
UniProt:Q96B36
Application:WB,IP
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY7054
数据表
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UniProt
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Mol Weight40kDa
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Alternative Names40 kDa proline rich AKT substrate; AKT1 S1; Lobe; PRAS40; Proline rich akt substrate; Proline rich Akt substrate 40 kDa;
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ImmunogenA synthesized peptide derived from human PRAS40
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionMany growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt. One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 protein. PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IP
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Recommended dilutionWB 1:500~1:2000 IP 1:50
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature. -
Western blot analysis of PRAS40 expression in (1) HeLa cell lysate; (2) RAW 264.7 cell lysate; (3) PC12 cell lysate.
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