Phospho-ERK1 (T202) + ERK2 (T185) Antibody_单克隆抗体-上海泊湾生物技术有限公司

Phospho-ERK1 (T202) + ERK2 (T185) Antibody

Source: Rabbit mAb Catalog No. : CY7165

Phospho-ERK1 (T202) + ERK2 (T185) Antibody (CY7165)
UniProt:P27361
Application:WB,IHC,ICC/IF,IP
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY7165

 Datasheet

数据表

UniProt

P27361
Mol Weight

42,44kDa
Alternative Names

MAPK3; MK03; MNK1; p44-ERK1; P44-ER; ERK-1; ERK1; ERT2; kinase ERK1; MAP kinase 1; MAPK 1;
Immunogen

A synthesized peptide derived from human Phospho-ERK1 (T202) + ERK2 (T185)
Storage

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Purification

Affinity-chromatography
Isotype

Rabbit IgG
Clonality

Monoclonal
Description

Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements.

Applications

Reactivity

Human,Mouse,Rat
Application

WB,IHC,ICC/IF,IP
Recommended dilution

WB 1:500~1:1000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:40

All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
Immunofluorescent analysis using the Antibody at 1:50 dilution.
Immunofluorescent analysis using the Antibody at 1:150 dilution.
Western blot analysis of Phospho-ERK1 (T202) + ERK2 (T185) expression in (1) NIH/3T3 cell lysate; (2) NIH/3T3 cell lysate treated with PDGF.