DNA Ligase IV Antibody
Source: Rabbit mAb Catalog No. : CY7178
DNA Ligase IV Antibody (CY7178)
UniProt:P49917
Application:WB,IHC,ICC/IF
Reactivity:Human
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY7178
数据表
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UniProt
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Mol Weight103kDa
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Alternative NamesDNA joinase; DNA ligase 4; DNA ligase IV; DNA repair enzyme; LIG4; LIG4S; Ligase IV;
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ImmunogenA synthesized peptide derived from human DNA Ligase IV
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionEfficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination.
Applications
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ReactivityHuman
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ApplicationWB,IHC,ICC/IF
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Recommended dilutionWB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200
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All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1k dilution for 1 hour at room temperature. -
Western blot analysis of DNA Ligase IV expression in Ramos cell lysate. -
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
Immunofluorescent analysis using the Antibody at 1:25 dilution. -
Immunofluorescent analysis using the Antibody at 1:25 dilution.
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