POLD1 Antibody
Source: Rabbit mAb Catalog No. : CY8250
POLD1 Antibody (CY8250)
UniProt:P28340
Application:WB,IHC,ICC/IF,FC
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY8250
数据表
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UniProt
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Mol Weight124kDa
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Alternative NamesCDC2; CRCS10; MDPL; POLD; POLD1;
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ImmunogenA synthesized peptide derived from human POLD1
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionPossesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF,FC
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Recommended dilutionWB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 FC 1:100
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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Western blot analysis of DNA Polymerase delta, catalytic subunit expression in (1) Jurkat cell lysate; (2) Raw264.7 cell lysate; (3) C6 cell lysate.



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