EIF4E Antibody
Source: Rabbit mAb Catalog No. : CY8863
EIF4E Antibody (CY8863)
UniProt:P06730
Application:WB,IHC,IP,FC
Reactivity:Human,Mouse,Rat
Source:Rabbit mAbhttp://www.abways.cn/showproduct.asp?cid=CY8863
数据表
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UniProt
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Mol Weight30kDa
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Alternative NamesCBP; eIF-4E; eIF-4F 25 kDa subunit; EIF4F; EIF4E1; EIF4EL1; MGC111573; EIF4E;
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ImmunogenA synthesized peptide derived from human EIF4E
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptioneIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,IP,FC
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Recommended dilutionWB 1:100~1:5000 IHC 1:100~1:500 IP 1:30 FC 1:40
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Western blot analysis of EIF4E expression in 293 cell lysate. -
Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:6K dilution) -
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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