Recombinant Galectin-3, Human ,AF
Source: Human Cytokines Catalog No. : PY2181
Recombinant Galectin-3, Human ,AF (PY2181)
UniProt:P17931
Application:Cell culture,Elisa
Endotoxin level:<0.1 EU per 1 μg of the protein by the LAL method.
Gene ID:Human Cytokineshttp://www.abways.cn/showproduct.asp?cid=PY2181
数据表
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UniProt
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Gene ID3958
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Calculated Molecular Weight27 kDa
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SDS-PAGE Molecular Weight(under reducing condition)32 kDa
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Alternative NamesIgE-binding protein, MAC2, L-29, CPB-35
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ActivityMeasured by its ability to agglutinate human red blood cells. The ED50 for this effect is <8 μg/mL.
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StorageLyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protei
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Purification>95% as determined by SDS-PAGE. Ni-NTA chromatography.
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Fusion tagHis-tag at the N-terminus
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Expression HostEscherichia coli
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DescriptionGalectin-3 is a protein that in humans is encoded by the LGALS3 gene. Galectin-3 is a member of the lectin family, of which 14 mammalian galectins have been identified. This protein has been shown to be involved in the following biological processes: cell adhesion, cell activation and chemoattraction, cell growth and differentiation, cell cycle, and apoptosis. Besides, Galectin-3 associates with the primary cilium and modulates renal cyst growth in congenital polycystic kidney disease.
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FormulationThe protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4.
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ReconstitutionIt is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Applications
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Endotoxin level<0.1 EU per 1 μg of the protein by the LAL method.
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ApplicationCell culture,Elisa
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Expression SequenceADNFSLHDALSGSGNPNPQGWPGAWGNQPAGAGGYPGASYPGAYPGQAPPGAYPGQAPPGAYPGAPGAYPGAPAPGVYPGPPSGPGAYPSSGQPSATGAYPATGPYGAPAGPLIVPYNLPLPGGVVPRMLITILGTVKPNANRIALDFQRGNDVAFHFNPRFNENNRRVIVCNTKLDNNWGREERQSVFPFESGKPFKIQVLVEPDHFKVAVNDAHLLQYNHRVKKLNEISKLGISGDIDLTSASYTMI with