Recombinant Galectin-7, Human ,AF
Source: Human Cytokines Catalog No. : PY2183
Recombinant Galectin-7, Human ,AF (PY2183)
UniProt:P47929
Application:Cell culture,Elisa
Endotoxin level:<0.1 EU per 1 μg of the protein by the LAL method.
Gene ID:Human Cytokineshttp://www.abways.cn/showproduct.asp?cid=PY2183
数据表
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UniProt
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Gene ID653499
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Calculated Molecular Weight15.9 kDa
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SDS-PAGE Molecular Weight(under reducing condition)18 kDa
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Alternative NamesLGALS7B, LGALS7, PI7; GAL7, Gal-7, HKL-14
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ActivityMeasured by its ability to agglutinate human red blood cells. The ED50 for this effect is <2 μg/mL.
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StorageLyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protei
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Purification>98% as determined by SDS-PAGE. Ni-NTA chromatography
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Fusion tagHis-tag at the N-terminus
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Expression HostEscherichia coli
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DescriptionThe galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-7 is a protein that in humans is encoded by the LGALS7 gene. It is expressed at all stages of epidermal differentiation (i.e., in basal and suprabasal layers). The protein was found mainly in stratified squamous epithelium. The cellular localization and its striking down-regulation in cultured keratinocytes imply a role in cell-cell and/or cell–matrix interactions necessary for normal growth control.
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FormulationThe protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4.
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ReconstitutionIt is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Applications
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Endotoxin level<0.1 EU per 1 μg of the protein by the LAL method.
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ApplicationCell culture,Elisa
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Expression SequenceSNVPHKSSLPEGIRPGTVLRIRGLVPPNASRFHVNLLCGEEQGSDAALHFNPRLDTSEVVFNSKEQGSWGREERGPGVPFQRGQPFEVLIIASDDGFKAVVGDAQYHHFRHRLPLARVRLVEVGGDVQLDSVRIF with polyhistidine tag at the N-terminus.



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