Recombinant HDGF (Hepatoma-derived growth factor), Human ,AF
Source: Human Cytokines Catalog No. : PY2190
Recombinant HDGF (Hepatoma-derived growth factor), Human ,AF (PY2190)
UniProt:P51858
Application:Cell culture,Elisa
Endotoxin level:<0.1 EU per 1 μg of the protein by the LAL method.
Gene ID:Human Cytokineshttp://www.abways.cn/showproduct.asp?cid=PY2190
数据表
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UniProt
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Gene ID3068
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Calculated Molecular Weight29.60 kDa
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SDS-PAGE Molecular Weight(under reducing condition)45 kDa
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Alternative NamesHMG1L2
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ActivityTesting in process
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StorageLyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protei
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Purification>98% as determined by SDS-PAGE. Ni-NTA chromatography
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Fusion tagHis-tag at the C-terminus
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Expression HostEscherichia coli
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DescriptionHDGF is a member of the hepatoma-derived growth factor family. HDGF plays a role as a secreted mitogen from the human hepatoma cell line Huh-7. HDGF is a nuclear targeted vascular smooth muscle cell mitogen as well as a heparin-binding protein that is greatly expressed in tumor cells where it stimulates proliferation. HDGF has several biochemical functions, for example, DNA binding, RNA polymerase II transcription corepressor activity, growth factor activity. Some of the functions are cooperated with other proteins, some of the functions could acted by HDGF itself.
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FormulationThe protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 7.4.
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ReconstitutionIt is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Applications
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Endotoxin level<0.1 EU per 1 μg of the protein by the LAL method.
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ApplicationCell culture,Elisa
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Expression SequenceMSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCVEEPEPEPEAAEGDGDKKGNAEGSSDEEGKLVIDEPAKEKNEKGALKRRAGDLLEDSPKRPKEAENPEGEEKEAATLEVERPLPMEVEKNSTPSEPGSGRGPPQEEEEEEDEEEEATKEDAEAPGIRDHESL with polyhisti



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