Recombinant MMP2 (active), Human ,AF
Source: Human Cytokines Catalog No. : PY2256
Recombinant MMP2 (active), Human ,AF (PY2256)
UniProt:P08253
Application:Cell culture,Elisa
Endotoxin level:<0.1 EU per 1 μg of the protein by the LAL method.
Gene ID:Human Cytokineshttp://www.abways.cn/showproduct.asp?cid=PY2256
数据表
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UniProt
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Gene ID4313
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Calculated Molecular Weight63.00 kDa
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SDS-PAGE Molecular Weight(under reducing condition)66 kDa
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Alternative NamesGelatinase A, TBE-1, MMP-II, MONA
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ActivityTesting in process
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StorageLyophilized protein should be stored at -20°C for 1 year. Upon reconstitution, store at 2°C to 8°C for up to 1 week. Further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1% BSA, 10%FBS, 5%HSA or 5% trehalose solution), protei
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Purification>95% as determined by SDS-PAGE. Ni-NTA chromatography.
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Fusion tagHis-tag at the C-terminus
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Expression HostEscherichia coli
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DescriptionMatrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. MMP2 (Matrix Metallopeptidase 2) is a Protein-Coding gene. Diseases associated with MMP2 include Multicentric Osteolysis, Nodulosis, and Arthropathy.
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FormulationThe protein was lyophilized from a 0.2 µm filtered solution containing 1X PBS, pH 8.0.
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ReconstitutionIt is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 100 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Applications
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Endotoxin level<0.1 EU per 1 μg of the protein by the LAL method.
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ApplicationCell culture,Elisa
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Expression SequenceMYNFFPRKPKWDKNQITYRIIGYTPDLDPETVDDAFARAFQVWSDVTPLRFSRIHDGEADIMINFGRWEHGDGYPFDGKDGLLAHAFAPGTGVGGDSHFDDDELWTLGEGQVVRVKYGNADGEYCKFPFLFNGKEYNSCTDTGRSDGFLWCSTTYNFEKDGKYGFCPHEALFTMGGNAEGQPCKFPFRFQGTSYDSCTTEGRTDGYRWCGTTEDYDRDKKYGFCPETAMSTVGGNSEGAPCVFPFTFLGNKYESC



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