COX IV Mouse mAb
Source: Mouse mAb Catalog No. : AB3119
COX IV Mouse mAb (AB3119)
UniProt:P13073
Application:WB,IHC,ICC/IF,IP,FC
Reactivity:Human,Mouse,Rat,Hamster,Goat,Monkey
Source:Mouse mAbhttp://www.abways.cn/showproduct.asp?cid=AB3119
数据表
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UniProt
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Mol Weight17KDa
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Alternative NamesCytochrome c oxidase subunit 4 isoform 1, mitochondrial; Cytochrome c oxidase polypeptide IV; Cytochrome c oxidase subunit IV isoform 1; COX IV-1; COX4I1; COX4;
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ImmunogenA synthetic peptide corresponding to carboxyl terminal residues of human COX IV
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StorageMouse IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeMouse IgG1
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ClonalityMonoclonal
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DescriptionThis protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport
Applications
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ReactivityHuman,Mouse,Rat,Hamster,Goat,Monkey
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ApplicationWB,IHC,ICC/IF,IP,FC
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Recommended dilutionWB 1:1000 IHC 1:50 ICC/IF 1:150 IP 1:100 FC 1:100
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Western blot detection of COX IV in Goat muscle,CHO-k1,COS7,3T3,Hela,C6 and K562 cell lysates using COX IV mouse mAb (1:5000 diluted).Predicted band size:17KDa.Observed band size:17KDa. -
Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma with COX IV Mouse mAb (4D11-B3-E8,1:50 diluted),showing cytoplasm localization.A high pressure mediated antigen retrieval step was performed in citrate buffer(pH6.0). -
Immunofluorescent analysis of HeLa cells using anti-COX IV mouse mAb diluted 1:150. -
Immunoprecipitation analysis of Hela cell lysates using COX IV mouse mAb. -
Flow Cytometry analysis of K562 cells stained with COX4 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.
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