LC3B Antibody
Source: Rabbit mAb Catalog No. : CY5992
LC3B Antibody (CY5992)
UniProt:Q9GZQ8
Application:WB,IHC,ICC/IF,IP
Reactivity:Human,Mouse,Rat
Source:Rabbit mAb
References（19）
http://www.abways.cn/showproduct.asp?cid=CY5992
数据表
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UniProt
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Mol Weight14,16kDa
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Alternative NamesATG8F; LC3B; MAP1LC3B; MLP3B; MAP1LC3B;
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ImmunogenA synthesized peptide derived from human LC3B
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StorageRabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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PurificationAffinity-chromatography
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IsotypeRabbit IgG
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ClonalityMonoclonal
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DescriptionUbiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production.
Applications
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ReactivityHuman,Mouse,Rat
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ApplicationWB,IHC,ICC/IF,IP
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Recommended dilutionWB 1:500~1:2000 IHC 1:50~1:100 ICC/IF 1:50~1:200 IP 1:50
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody at 1:50 dilution. -
Immunohistochemical analysis of paraffin-embedded Human testis cancer, using the Antibody at 1:200 dilution. -
Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma, using the Antibody at 1:50 dilution. -
Immunofluorescent analysis using the Antibody at 1:150 dilution. -
Western blot analysis of LC3B expression in (1) Human brain lysate; (2) RAW 264.7 cell lysate; (3) C6 cell lysate. -
Immunofluorescent analysis of Hela cells treated with choroquine, using LC3B Antibody. -
Prostaglandin E1 Inhibited Diabetes-Induced Phenotypic Switching of Vascular Smooth Muscle Cells Through Activating Autophagy. -Cellular Physiology and Biochemistry -
SIRT 5‐mediated deacetylation of LDHB promotes autophagy and tumorigenesis in colorectal cancer. -Molecular Oncology -
Prostaglandin E1 Inhibited Diabetes-Induced Phenotypic Switching of Vascular Smooth Muscle Cells Through Activating Autophagy. -Cellular Physiology and Biochemistry -
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature. -
Immunoprecipitate (IP) analysis using the Antibody at 1:50 dilution. (wb at 1:6K dilution)
引用文献（19条）
Prostaglandin E1 Inhibited Diabetes-Induced Phenotypic Switching of Vascular Smooth Muscle Cells Through Activating Autophagy.-Cellular Physiology and Biochemistry
Dual receptor recognizing liposomes containing paclitaxel and hydroxychloroquine for primary and metastatic melanoma treatment via autophagy-dependent and independent pathways.-Journal of Controlled Release
SIRT 5‐mediated deacetylation of LDHB promotes autophagy and tumorigenesis in colorectal cancer.-Molecular Oncology
Size-Adjustable Micelles Co-loaded with Chemotherapeutic Agent and Autophagy Inhibitor for Enhancing Cancer Treatment via Increasing Tumor Retention.-Acta Biomaterialia
Impaired autophagic flux contributes to muscle atrophy in obesity by affecting muscle degradation and regeneration-Biochemical and Biophysical Research Communications (BIOCHEM BIOPH RES CO)
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